WELLNESS FROM WITHIN
Marsing & Co. Africa are now a proud sole supplier of the Wellnex brand in South Africa
A breath of life for women and, hope for the elderly, or energy for the young and old. As a gemstone of beauty and health, we continue to improve in order to enhance your lives.
Nitta Gelatin presents Wellnex functional peptides. Their hidden, undiscovered potential will change the lives of tomorrow as well as the standards of the future.
“To stay healthy and feel young”.
*Wellnex is a term coined by combining the word “wellness” and the symbol “x” and represents multiple possibilities.
COLLAGEN THE BUILDING PROTEIN
The human body is made up of protein, fat and carbohydrate. Collagen is a type of protein largely found in the skin, and that accounts for the
30% of the total protein content in the body.
Collagen* constitutes 90% of the total protein found in the bones.
Bone descriptions have compare calcium to concrete while collagen has been referred to as the steel frame.
Loss of collagen leads to degeneration of cartilage, wrinkled skin and brittle bones etc.
*Collagen refers to type 1 collagen.
WHY COLLAGEN IS IMPORTANT?
– Collagen is a cellular scaffold and also acts as a lubricant that facilitates intercellular exchange of diverse
substances owing to its ability to fill intercellular spaces.
– Ageing is associated with collagen degradation and loss, a process that can lead to cellular death.
– However, recent studies indicate that cells can produce collagen following stimulation with collagen-derived active ingredients.*
Participating subjects included 74 humans between 15 and 93 years of age. Both of these values begin to decrease sharply around the age of 20 years.
Source: Shustor et al., Reference 11
WHAT IS COLLAGEN PEPTIDE?
COLLAGEN UBIQUITOUS PROTEIN
Key amino acids for skin health
ALL COLLAGEN PEPTIDES ARE NOT THE SAME
Figure 1 – Intestinal absorption of amino acid and peptide.
Levels of collagen Hyp peptides in the blood following oral ingestion
BEAUTY FROM WITHIN
Supple skin | Improved viscoelasticity | Reduced wrinkles
o Rebuilds cartilage
o Prevents cartilage degeneration
o Improves bone mineral density
o Strengthens tendons and ligaments
o Reduces pain related osteoarthritis
CLINICAL FINDINGS
Decrease in the number of hidden blemishes
Clinical Trial
o Randomized, Placebo controlled, double blind
o 39 female subjects aged 35 to 55 years with skin problems
o Wellnex: 26 subjects
o Placebo: 13 subjects
o Dosage: 5g/day for 8 weeks
o Evaluation: Hydration, elasticity, roughness
o Country: Japan
Women who consumed collagen peptides showed a significant decrease in hidden blemish scores compared to the placebo group.
“Clinical effects of collagen hydrolysates ingestion on UV induced pigmented spots
of human skin” Fumihito Sugihara, Naoki Inoue, Health Sciences Vol. 28, No.s, 2021,
page 153
Clinical Trial
o Randomized, Placebo controlled, double blind
o 56 female subjects aged 30 to 55 years with skin problems
o Wellnex: 28 subjects
o Placebo: 28 subjects
o Dosage: 2.8g/day for 8 weeks
o Evaluation: Hydration, elasticity, roughness
o Country: China
Significant Improvement in elasticity, moisture and smoothness within 8 weeks.
Sugihara F, Inoue N, Wang X: Clinical effects of ingesting collagen hydrolysate on facial skin properties, Jpn Pharmacol Ther; 43:67-70, 2015
Clinical Trial
o Randomized, Placebo controlled, double blind
o 71 female subjects aged 30 to 55 years with skin problems
o Wellnex: 37 subjects
o Placebo: 34 subjects
o Dosage: 3.0g/day for 12 weeks, 20ml beverage
o Evaluation: Hydration, elasticity, roughness
o Country: South Korea
Significant Improvement in elasticity, moisture and smoothness within 8 weeks.
Seiko Koizumi, Naoki Inoue, Maiko Shimizu, Change-ju Kwon, Hwa-young, Kim & Kyoung Sik Park “Effects of Dietary Supplementation with Fish Scales-Derived Collagen Peptides on Skin Parameters and Condition: A Randomized, Placebo-Controlled, Double-Blind Study” International Journal of Peptide Research and Therapeuticsm DOI 10.1007/s10989-017-9626-0
Knee Join Osteoarthritis (India)
Knee Join Osteoarthritis (India)
o Collagen peptide (BBCP; Mw5,000)
o 5g x 2 per day dose
o N=10 for placebo group, N=20 for collagen peptide group with knee joint
osteoarthritis patient
o 14 Weeks study
o Measured WOMAC, VAS and QOL score data
Osteoporosis
Randomised, Open Label, Multi Center, Two Arms Parrallel Group
o Collagen peptide (BBCP; Mw5,000)
o Age: 45 – 55 years
o 10g Per day dose for 1 year
o Wellnex = 31 subjects,
comparator – 34 subjects
o Evaluation: Dexa bone scan | Serum
BAP | Serum OsteoCalcin
o Randomized clinical trial.
o Test materials: AAA Ca, 300mg, Collagen Peptide 3500mg, Composite
Mucopolysaccharide 200mg, Glucosamine 600mg, Vitamin C 200mg, Glucose,
Maltose, citric acid, lemon juice, lemon flavour and acesulfan.
Dosage:
o Supplementation group: Ca-Collagen-Matrix, dissolved in warm water, 3 times/day,
after meal for 4 months + basal treatments before and after study period.
o Control group: Only Basal treatment.
o Measurement: Pain was assessed subjectively by the face scale and objectively by
measuring skin impedance.
o Skin impedance measured in quiet sitting position and after applying loads to the
spine and knee joints.
Conclusion:
o Marked alleviation of subjective pain in supplementation group.
o A fall of skin impedance was also alleviated in supplementation group.
o Basal skin impedance was significantly reduced (usually increase with age)
in supplementation group.
IDEAL SUPPLEMENT FOR ACTIVE & SPORTS PEOPLE
Support athletic performance
MANAGING DIABETES THE HEALTHY WAY
Clinically proven to control diabetes
o Ideal healthy intervention in early stages of diabetes.
o Add on effect with regular medical therapy.
o Improvement in quality of life.
o Enhance joint and bone health.
o Significant reduction in FBS and HbA1C.
o Significant improvement in insulin sensitivity.
o Synergic effect with regular medical therapy.
HEALTHY SOLUTION FOR WELLNESS
WHAT MAKES WELLNEX UNIQUE?
o Formulation development and sharing formulation.
o Different grades for powder and beverages.
o Market trends from developed countries.
o Customized product solutions.
o Retail packaging.
o Clinical evidence – functional.
COLLAGEN BOOM
Japan is the largest consumer of collagen in the world. In Japan, collagen peptides are primarily valued for their cosmetic properties. Globally, collagen peptides are consumed
for their effects on joint health and for nutritional purposes, thus reflecting the diversity of beneficial properties associated with collagen peptide use.
80% Is beauty, collagen bible.
COMPATIBLE IN ALL DELIVERY FORMATS
Drink Shots
Jellies, Gummies, Tables
Powder (Sachets and Jars)
| Grade/Type | Bronze/BCG50NR | Silver/BCG50SP | Gold/BCG50HG | Platinum/BCG30HG | Drink Grade/BCP30DR |
|---|---|---|---|---|---|
| Protein % (Dry basis) | > 98 | > 98 | > 98 | > 98 | > 90 |
| Molecular Weight (Dalton) | ~ 5000 | ~ 5000 | ~ 5000 | ~ 3000 | ~3000 |
| Dulk Density (g/m) | 0.28 - 0.32 | 0.28 - 0.32 | 0.28 - 0.32 | 0.28 - 0.32 | 0.30 - 0.35 |
| Dissolution Time (Seconds) | 130 - 150 | 130 - 150 | 120 - 150 | 90 - 120 | 100 - 150 |
| Dispersion Time (Seconds) | < 50 | < 50 | < 50 | < 20 | < 50 |
| Clarity of Solution (10% Solution) | > 17 | 13 - 16 | < 10 | < 9 | < 9 |
| Odour | Strong metallic/chemical topnotes | Metallic/chemical topnotes | Mild metallic/chemical topnotes | Very mild metallic/chemical topnotes | Very mild metallic/chemical topnotes |
| Taste | Tart notes, sharp bovine aftertaste | Tart notes, sharp aftertaste | Palatable, smooth flavour | Tastes best among three variants | Citric sour taste |
| Heavy Metals | < 50 ppm | < 50 ppm | < 50 ppm | < 50 ppm | < 50 ppm |
| Particle Size | # 10 pass | # 10 pass | # 10 pass | # 20 pass, # 100 pass | # 100 pass |
| Packaging | 10Kg paper bag | 10Kg paper bag | 10Kg paper bag | 10Kg paper bag | 15Kg paper bag |
SCIENTIFICALLY PROVEN
Domestic patents
JPB 3574612 – Collagen supplemented to food and drinks
JPB 3881453 – Calcium absorption-enhancing collagen peptides and their manufacturing method
JPB 4377522 – Method of manufacturing of stable, acidified milk beverage using collagen peptides
JPB 4490498 – Disease-suppressing agent (bones, joints and wound healing)
JPB 4523339 – Agent that enhances bone strength
JPB 4709730 – Chewable tablet
JPB 5361155 – Degraded gelatin powder and its manufacturing method
Registered trademark
No. 5524071 – Wellnex
1. Liu C, Sugita K, Nihei KI, Yoneyama K, Tanaka H: Absorption of hydroxyproline-containing peptides in vascularly perfused rat small intestine in situ. Biosci Biotech Bioch;
73:1741-1747, 2009
2. Nakatani S, Mano H, Sampei C, Shimizu J, Wada M: Chondroprotective effect of the bioactive peptide prolyl-hydroxyproline in mouse articular cartilage in vitro and in vivo.
Osteoarth Cartilage; 17:1620-1627, 2009
3. Sugihara F, Inoue N, KuwamoriM, Taniguchi M: Quantification of hydroxyprolyl-glycine (Hyp-Gly) in human blood after ingestion of collagen hydrolysate. J Biosci Bioeng;
113:202-203, 2012
4. Sugihara F, Inoue N: Clinical effects of collagen hydrolysates ingestion on UV-induced pigmented spots of human skin: A preliminary study. Health Sciences; 28:153-156, 2012
5. Nakatani S, Kobata K, Nakajima H, Kimira Y, Mano H, Sugihara F, Wada M: Transcriptome of ATDC5 Cultured with Glucosamine Hydrochloride and Collagen Hydrolysate Indicates
a New Candidate Gene for the Differentiation of Chondrocytes. J Chitin Chitosan Sci; 2:233-237, 2014
6. Kumar S, Sugihara F, Suzuki K, Inoue N, Venkateswarathirukumara S: A double-blind, placebo-controlled, randomised, clinical study on the effectiveness of collagen peptide on
osteoarthritis. J Sci Food Agric; 95:702-707, 2014
7. Kimira Y, Ogura K, Taniuchi Y, Kataoka A, Inoue N, Sugihara F, Nakatani S, Shimizu J, Wada M, Mano H: Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation
of MC3T3-E1 osteoblastic cells. Biochem Biophys Res Co; 453:498-501, 2014
8. Sugihara F, Inoue N, Wang X: Clinical effects of ingesting collagen hydrolysate on facial skin properties. Jpn Pharmacol Ther; 43:67-70, 2015
9. Sugihara F, Inoue N, Koizumi S, Sriraam VT: Collagen hydrolysate enhanced pressure ulcer healing in a randomized double-blind placebo-controlled clinical study.
Jpn Pharmacol Ther; 43:1323-1328, 2015
10. Inoue N, Sugihara F, Wang X: Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-
blind placebo-controlled clinical study. J Sci Food Agric; 2016, in press
11. Castelo-Branco C, Pons F, Gratac E, Fortuny A, Vanrell JA, Gonz疝ez-Merlo J: Relationship between skin collagen and bone changes during aging. Maturitas; 18:199-206, 1994
12. Shigemura Y, Iwai K, Morimatsu F, Iwamoto T, Mori T, Oda C, Taira T, Park EY, Nakamura Y, Sato K: Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in
human blood, on growth of fibroblasts from mouse skin. J Agric Food Chem; 57:444-449., 2009
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acid synthesis in cultured human dermal fibroblasts. Journal Dermatol 37:330-338, 2010.
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hydrolysates from different sources. J Agric Food Chem; 55:1532-1535, 2007
15. Inoue K, Nakamura I, Takasaki M, Maeda R, Saito I, Maeda K, Anaka Y: Nutrition intervention methods based on home-visit nutritional support and verification of the resulting
improvement effects. J Jpn Diet Assoc; 55:656-664, 2012
16. Ichimura T, Yamanaka A, Otsuka T, Yamashita E, Maruyama S: Antihypertensive effect of enzymatic hydrolysate of collagen and Gly-Pro in spontaneously hypertensive rats.
Biosci Biotech Bioch; 73:2317-2319, 2009; Food Sci Technol Res; 2009